PHENOLIC ACIDS OF PLANT ORIGIN AS A PROMISING SOURCE FOR THE DEVELOPMENT OF ANTIVIRAL AGENTS
Aizhan S TURMAGAMBETOVA, https://orcid.org/0000-0002-7878-6133,
Andrey P BOGOYAVLENSKIY, http://orcid.org/0000-0001-9579-2298,
Pavel G ALEXYUK, https://orcid.org/0000-0003-3638-3341,
Madina S ALEXYUK, https://orcid.org/0000-0003-3479-4438,
Vladimir E BEREZIN, https://orcid.org/0000-0002-2220-5758
Research and Production Center for Microbiology and Virusology, Almaty, Republic of Kazakhstan
Actuality. Seasonal outbreaks of respiratory infections leading to severe complications and high mortality are characteristic of the influenza virus type A. ,Various etiotropic antiviral agents have been developed since the middle of the last century against influenza epidemics and pandemics. The rapid adaptation of the influenza virus and the emergence of drug-resistant strains are the serious problems in the influenza infection treatment. Therefore, the effectiveness of newly developed drugs is determined based on an assessment of their interaction with drug-resistant influenza virus strains. Phenolic acids of plant origin are promising compounds for the development of antiviral agents with a wide spectrum of biological activity and are less susceptible to the emergence of resistant to them strains.
The aim of this work was a comparative study of the antiviral activity of four phenolic acids: Gallic, Syringic, Vanillic and Protocatechuic against influenza A virus in in ovo experiments.
Material and methods. In the present work on the model of two drug-resistant strains of the influenza A virus a comparative study of the antiviral activity of four plant origin phenolic acids: Gallic, Syringic, Vanillic and Protocatechuic is carried out.
Results and discussion. It was shown that Gallic acid is able to inhibit the reproduction of influenza virus A/H5N3 by 50% at a concentration of 1.35 μg/mL; and at a concentration of 0.1 μg/mL is able to inhibit the 50% reproduction of influenza virus A/H3N2, which is comparable with the activity of last-generation anti-influenza drug Tamiflu.
Conclusion. It was shown that for the pronounced antiviral activity phenolic acid must have at least two hydroxyl groups in molecule. The replacement of hydroxyl groups to methoxy leads to an almost complete loss of antiviral properties by phenolic acid. The gallic acid is a potential candidate for the development of promising agents for the treatment of influenza A virus.
Keywords: influenza, Gallic acid, Protocatechuic acid, antiviral activity.